Figure 5.

STAT3KO mice induce expansion of IL-10-producing regulatory CD8⁺ T cells during EAU. (a–d) WT or STAT3KO mice were immunized with IRBP in CFA. (a) Fundus images taken on day-21 after immunization. (b) and 21 days after immunization. Freshly isolated LN cells (21 days after immunization) were analyzed by the intracellular cytokine staining (b, c). CD3⁺ T cells were gated, and numbers in quadrants indicate percentage of CD4⁺ or CD8⁺ T cells expressing IL-17A or IL-10. (d, e) CD8⁺ T cells were isolated from the LN by magnetic sorting, and RNA was analyzed for IL-10 expression by RT-qPCR (d) or RT-qPCR (e). Data represent at least 3 independent experiments.