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Western blot analysis for the presence of exosomal marker proteins CD63 and CD9 in HeLa cell culture media ((a), (b)) and serum ((c), (d)) derived samples. Exosomes isolated with either the Total exosome isolation reagents or ultracentrifugation protocols were separated on 4–20% Tris-Glycine gels. Standard Western blot procedures with anti-CD63 and anti-CD9 antibodies were used to detect exosomal protein markers.
