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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Biomaterials. 2013 Jul 1;34(30):7227–7235. doi: 10.1016/j.biomaterials.2013.05.079

Fig 4.

Fig 4

Spheroid merging enables local control of mesoderm induction. A) One day after aggregate formation, a second spheroid population was added containing fluorescently labeled MPs. After an additional day of culture, spheroids had merged in the microwells (arrows). B) A merged spheroid in suspension fabricated from an untreated spheroid and a spheroid containing optically dense MPs. C) The merging of untreated spheroids resulted in few GFP+ cells dispersed throughout individual aggregates, whereas D) addition of soluble BMP4 promoted GFP+ cells throughout merged aggregates. E) Soluble BMP4 addition to spheroids containing unloaded heparin–gelatin MPs resulted in some GFP localization mostly within the hemisphere containing the MPs. F) The merging of untreated aggregates with aggregates containing BMP4 loaded heparin–gelatin MPs yielded GFP+ cells localized predominantly within The hemisphere of the spheroid containing the MPs. Scale bars = 100 µm (A,B), 200 µm (C–F).