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. Author manuscript; available in PMC: 2013 Oct 20.
Published in final edited form as: Gene. 2005 Jul 18;354:132–139. doi: 10.1016/j.gene.2005.03.028

Fig. 3.

Fig. 3

Steady-state levels of pro- and anti-apoptotic proteins and caspases in etoposide-treated control and mtDNA-depleted C2C12 cells. Cells treated with (25 µM for 4 h) or without etoposide were used for isolating the mitochondrial and cytosolic fractions as described in the Materials and methods section. 30 µg each of mitochondrial (A and B) and cytosolic (C) proteins was subjected to Western blot analysis using indicated antibodies. The immunoblot was developed using the Pierce Super signal West Femto substrate kit as described in the Materials and methods.