Figure 3.

A, Immunoblots of lipogenesis-related and insulin signaling-related proteins (panel C) in total lysates from WAT of lean and OB dams at PND21 (n = 4 pools per group from 7 litters for lean and 5 litter for OB). B and D, Densitometric quantification of blots. E, Immunoblots of WAT lysates 5 minutes following acute insulin challenge administered through the hepatic portal vein (10 U/kg, n = 4–7; see Supplemental Table 1 for litters). Statistical differences were determined using Student's t test. *, Significance P < .05. ACC, acetyl-CoA carboxylase; ADU, arbitrary density units; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.