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. 2013 Aug 8;154(11):4249–4258. doi: 10.1210/en.2013-1274

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Copyright © 2013 by The Endocrine Society

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Figure 3. — (A–F) Confocal images (1-μm optical section) through the ovine ARC processed for dual immunofluorescence detection of OFQ (red) and either POMC, kisspeptin, AgRP, or TH (green). (C) Merged image of single-labeled OFQ neurons (A) and POMC neurons (B) to depict yellow dual-labeled OFQ/POMC neurons (eg, arrows). Note presence of a few single-labeled POMC neurons (eg, top-middle portion of B and C). Merged images in bottom row show OFQ and kisspeptin (Kiss) (D), AgRP (E), or TH (F). Magnification bar, 25 μm.