Table 2.

Microscope lens and confocal laser scanning microscopy (CLSM) settings used for the observation of the specimens; Ch1 and Ch2 = detection channels 1 and 2.

Lens	HC PL APO CS (High-grade colour-corrected Plan Apochromat lens for confocal)
Objective	20×
Numerical aperture	0.7
Immersion	Oil
Excitation wavelength	488 and 633 nm
Laser intensity	50% and 33%, respectively
Excitation beam splitter	TD 488/561/633
Detected emission wavelength	Ch1: 493 – 600 nm
	Ch2: 650 – 750 nm
Detector gain	833.8 and 791.6 V
Amplitude offset	-0.9 and -1.0 %
Electronic zoom	3X
Pinhole aperture	54.6 μm
Image format	2048 × 2048 dpi