Fig. 1.

Induction of endogenous AQP2 expression and apical trafficking in mpkCCD clone 11 cells using dDAVP. (A) Experimental protocol. (B) Immunoblotting for AQP2 levels at various stages in the experimental protocol. g-AQP2, glycosylated AQP2; I, II, III, and IV, protein samples collected at various experimental stages as in A; IM, mouse kidney inner medulla lysate; ng-AQP2, nonglycosylated AQP2. (C) Confocal immunofluorescence staining of AQP2. ZO1, zonula occludens protein 1. (D) Immunoelectron microscopic staining of AQP2. Arrow, AQP2; N, nucleus.