Fig. 4.

Functional validation of ZmCCT via transformation. (A) The DNA fragment used for the ZmCCT complementation test is shown. (B) The greenhouse performance of T₀ transgenic plants. Red arrows indicate transgenic plants. (C) The greenhouse performance of T₂ transgenic plants. Transgenic plants and sibling controls are indicated by (+) and (−), respectively. (D) Comparison between transgenic lines and controls in T₀ and T₂ generations under long-day greenhouse conditions. DTA, days to anthesis (for T₀ plants, from the date of transplantation to anthesis); EH, ear height; PH, plant height; TLN, total leaf number. Data are shown as mean ± SE.