Fig. 1.

Arf6 interaction with the WAVE regulatory complex. (A) Proteins recruited from extract to phospholipid bilayers by Arf1 and Arf6. Silica beads coated with phospholipid bilayers (membrane platforms depicted in cartoon) anchored with inactive GDP-bound or active GTPγS-bound myristoylated Arf1 or Arf6 (green circles) isolated from extract. Recruited proteins (labeled at right and marked with orange circles) were extracted and analyzed by SDS/PAGE and Coomassie blue staining. Molecular weight markers are in kilodaltons (Left). Asterisk marks a recruited extract protein migrating above recombinant Arfs. (B) Parallel immunoblotting of samples from A with indicated antibodies (Right). (C) Recruitment of Arf binding domain (GAT, orange circles) of GGA3 to membrane platforms with anchored Arf1 or Arf6 (green circle) in buffer.