Fig. 1.

Chromosomal patterns of 5mC and 5hmC content in mouse hair follicle DSCs under conditions that promote nonrandom sister chromatid segregation. Shown are epifluorescence and phase-contrast micrographs of 3C5 cells grown under Xn-free conditions to promote ASR with nonrandom segregation. Fluorescence micrographs compare nuclear DNA fluorescence (DAPI), in situ immunofluorescence images with either anti-5mC antibodies (A) or anti-5hmC antibodies (B), and overlaid images (Merge in A and B). Examples of symmetric (SYM) and asymmetric (ASYM) patterns of chromosomal 5mC and 5hmC content from SPr, CD, and mitotic cell analyses are compared. (Scale bars, 25 microns.)