Fig. 4.
Higher 5hmC content marks immortal DNA chromosomes in mouse hair follicle DSCs undergoing nonrandom segregation. Shown are epifluorescence and phase-contrast micrographs of cytochalasin D-arrested binucleated 3C5 and 5B3 cells at the completion of a BrdU label retention-CD study with culture under Xn-free conditions to promote ASR with nonrandom segregation. Fluorescence micrographs compare nuclear DNA fluorescence (DAPI), serial ISIF images with anti-BrdU and anti-5hmC antibodies, and overlaid images (Merge). Examples of cosymmetric (Co-Sym) and coasymmetric (Co-Asym) patterns of BrdU and 5hmC chromosomal content are shown. (Scale bars, 25 microns.)