Fig. 6.

IL-10 plays a crucial role in Tr1-like cells-mediated systemic tolerance. (A and B) Treatment was performed as in Fig. 3E; IL-10 and IFN-γ levels were determined in culture supernatants of sorted CD4⁺ T cells from liver MNCs of control, HBV-carrier, or IL-10^−/−HBV mice. (C) GC B-cell frequency was measured in DLN from HBV-carrier or IL-10^−/− HBV-injected mice after HBsAg vaccination. (D) Splenic CD4⁺ T cells and splenocytes without CD4⁺ T cells (called non-CD4⁺ cells), from WT, HBV-carrier, or IL-10^−/− HBV mice, were transferred to recipient Rag1^−/− mice in various combinations. ND, not detected. Results represent two independent experiments (n = at least 3 per group). *P < 0.05.