Fig. 4.

CD94 is a peptide-specific receptor for HLA-E. (A) Clustering of CD94 on the surface of Jurkat–CD94 transfectants in the presence of the indicated peptides. (B and C) Fold increase in intensity (B) and percentage of aggregates with conjugates from the Jurkat–CD94 clustering experiments (C). Overall, 40–60 conjugates were analyzed for each condition. Data are shown as mean + SEM of three independent experiments. (D) Pentamer binding of HLA-E and HLA leader peptide to NKL but not to Jurkat–CD94 transfectants. The mean fluorescence intensity of each histogram is indicated. Statistical analyses were performed using ANOVA with Dunnett’s post test to compare individual pairs of conditions. **P < 0.01; ***P < 0.001; ns, nonsignificant.