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. 2013 Sep 30;110(42):16981–16986. doi: 10.1073/pnas.1304366110

Fig. 5.

Fig. 5.

NKG2A+ but not KIR+ NK cells are tuned to low cell-surface levels of MHC class I. .174 cells were incubated with increasing concentrations of HLA-Gsp or VAPWNSFAL (VAP-FA) before the stabilization of HLA-E (A) or HLA-Cw*0102 (B) was measured. They were then used as targets in degranulation assays for (A) NKG2A+ CD158b− (NKG2A+) or (B) NKG2A− CD158b+ (KIR2DL2/3+) NK cells. The levels of cell-surface MHC class as determined by flow cytometry and the fraction of degranulating NK cells were then correlated. The data for MHC class I stabilization are normalized to “no peptide” (0% stabilization) and “100 μM peptide” (100% stabilization) concentrations. For degranulation, data are normalized to CD107a expression for .174 cells incubated in the absence of peptide (0% inhibition) or the presence of 100 μM peptide (100% inhibition).