Table 1.

Genes affected by the second wave of STAT3 activation

Gene	Fold induction compared with untreated cells
	7 h	7 h + Ab	7 h − EGFR	12 h	12 h + Ab	12 h − EGFR
ABCB1	1.4	0.9	1.0	2.0	1.1	1.1
CDK5RAP3	1.7	0.7	0.2	2.7	1.3	0.5
FOS	7.7	0.9	2.3	3.0	1.0	1.9
GADD45G	2.0	1.0	3.3	1.7	1.1	2.7
HDAC4	1.4	1.2	1.6	2.4	1.4	1.9
IGSF3	1.1	1.8	0.8	2.0	1.1	1.0
IRAK1BP1	1.1	1.2	2.4	1.6	1.0	1.7
IRF1	2.2	1.3	1.8	1.9	1.4	1.3
IRF8	1.6	0.7	1.1	1.9	1.2	0.9
MAPK8	1.8	0.8	6.0	1.2	1.0	6.8
MLH3	2.3	0.4	0.1	1.6	1.1	0.6
MUC1	3.5	3.5	2.3	4.9	1.9	1.9
MUC6	1.3	0.8	1.3	1.8	0.6	0.6
PRKAA1	1.7	1.0	0.9	1.6	1.5	1.0
SAA1	2.4	1.2	11.4	2.6	1.6	15.3

See the legend to Fig. S1 for the protocol. DLD1 cells were treated with IL-6 for 30 min, followed by washout and replacement of the medium, with or without anti–IL-6. Cells in which EGFR was knocked down were also used, without exposure to anti-Il-6 antibody (Ab). Cells were collected for analysis 7 or 12 h after washout. The expression of 47,323 probes on an Illumina HumanHT-12 v4 Expression BeadChip was analyzed. The average signal for each probe was used to determine expression levels. Genes with average signals below 25 and detection P values greater than 0.01 in the treated cells were excluded from the analysis. The numbers in the table are fold inductions relative to untreated control cells. Inductions of less than 1.5-fold were not scored, and reductions of induction of less than twofold in response to anti–IL-6 were also not scored. SAA1 was not detected in untreated shEGFR cells but was induced in response to IL-6; the numbers are the fold inductions compared with the levels of expression in untreated shNT (nontargeting shRNA) cells.