Skip to main content
. Author manuscript; available in PMC: 2014 Oct 15.
Published in final edited form as: J Immunol. 2013 Sep 18;191(8):4456–4465. doi: 10.4049/jimmunol.1300827

Figure 2. Phenotypic analysis of lymph node cells associated with gp96A488.

Figure 2

Lymph nodes from unimmunized or gp96 immunized mice were analyzed by multi-color flow cytometry. (A) At 8 hours post immunization with 10 μg gp96A488, total lymph node and gp96+ cells were phenotyped for CD11c and CD11b expression. The CD11c population was CD11b+/− and CD11b+ population was CD11c+/−. (B) A488+CD11b+ lymph node cells were additionally phenotyped for CD4, CD8, Gr1.1, F4/80 and MHC II. (C) A488+CD11c+ lymph node cells were additionally phenotyped for CD4, CD8, CD207, CD103 and MHC II. At 8 (D) or 48 (E) hours post immunization with 10 μg gp96A488, lymph nodes were harvested and phenotyped for the indicated populations. The distribution of gp96 in each population is indicated as a percentage and compared to the percentage of that population in the total lymph node. Percentages are the average from 5 mice, and representative of 2 experiments. Data are represented as mean +/− SEM.