Figure 1.

Non-interdependence of STN kinase accumulation. (A) WT (Col-0) thylakoid proteins corresponding to 10 μg (2.0×) and 5 μg (1.0×) of Chl and serial dilutions of oeSTN8 thylakoids were loaded to quantify the levels of STN8 protein in oeSTN8 plants. Proteins were subjected to SDS-PAGE and Western blot analysis using STN8- and Lhca3-specific antibodies. (B) STN7 accumulation in thylakoids of WT (Col-0), oeSTN8 and stn8-1 mutant plants. Thylakoid proteins, corresponding to 8 μg (100%), 4 μg (50%), and 2 μg (25%) of Chl, from each genotype were loaded on a SDS gel and probed using specific antibodies against STN7 and Lhca3 (as loading control). (C) Accumulation of STN8 in thylakoids of WT (Col-0) and oeSTN7. Thylakoids of WT corresponding to 5 μg of Chl and serial dilutions of oeSTN7 thylakoid proteins were separated by SDS-PAGE, and immunoblots were probed with antibodies specific for STN8 and Lhca3 (as loading control). (D) Thylakoids of WT (Col-0), stn8-1, stn7-1, oeSTN8, and oeSTN7 plants corresponding to 5 μg of Chl were analyzed by Western blotting with antibodies raised against an STN8-specific (left panel) or an STN7-specific (right panel) peptide fragment. Replicate SDS-gels were stained with Coomassie brilliant blue (CBB) and the section with the LHCII signal is shown as loading control.