Figure 3.

Cytokine-producing T cells from peripheral blood. Peripheral blood mononuclear cells (PBMCs) isolated from non-segmental vitiligo (NSV) patients and healthy controls were stimulated with phorbol myristate acetate (PMA) and ionomycin for 5 h in the presence of Golgi stop. Then, the IFNγ- and IL-4-producing T cells were determined by intracellular staining and flow cytometry analysis. (A) Representative FACS dot plots for IL-4- and IFN-γ-producing CD4⁺ and CD8⁺ T cells gated on CD4-positive and CD8-positive cells. (B) Summary plots showing individual results of the frequency of IL4- and IFN-γ-producing CD4⁺ and CD8⁺ T cells in NSV patients versus healthy controls.