Table 5.

Kinesin-5 velocity, measured by single molecule experiments.

Kinesin-5isoform	Oligomeric State	Buffer (pH)	Salt/Other (Osmolyte)	Velocity (nm/s)	Reference
Single-molecule measurements (trap)
X. laevis	native tetramer	80 mM PIPES (6.8)	-	35.0	(Korneev et al., 2007)
X. laevis	chimeric dimer (1-373 with KHC 345-426)	80 mM PIPES (6.8)	-	59.0	(Duselder et al., 2012)
H. sapiens	native dimer (1-513)	80 mM PIPES (6.9)	200 mM KCl, 50 mM K-acetate	68.0	(Valentine and Block, 2009)
H. sapiens	native dimer (1-513)	80 mM PIPES (6.9)	200 mM KCl, 50 mM K-acetate	96.0	(Valentine et al., 2006)
Single-molecule measurements (fluorescence)
X. laevis	native tetramer	80 mM PIPES (6.8)	-	14.6	(Kwok et al., 2006)
X. laevis	native tetramer	70 mM PIPES (6.8)	-	8.5	(Weinger et al., 2011)
X. laevis	native tetramer	70 mM PIPES (6.8)	-	8.9	(Kapitein et al., 2008)
X. laevis	native tetramer	70 mM PIPES (6.8)	20 mM KCl	10.1	(Kapitein et al., 2008)
X. laevis	native tetramer	70 mM PIPES (6.8)	40 mM KCl	0.0	(Kapitein et al., 2008)
X. laevis	native tetramer	70 mM PIPES (6.8)	60 mM KCl	0.3	(Kapitein et al., 2008)
X. laevis	chimeric dimer (1-373 with KHC 345-426)	80 mM PIPES (6.8)	-	112.0	(Duselder et al., 2012)