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. Author manuscript; available in PMC: 2013 Oct 21.
Published in final edited form as: Brain Res. 2009 Jan 30;1265:138–147. doi: 10.1016/j.brainres.2009.01.033

Fig. 6. Effects of Rac1 AS-ODNs or Rac1 inhibitor (NSC23766) on delayed apoptotic neuronal death by histology analysis.

Fig. 6

(A) Cresyl violet and TUNEL staining was performed on the sections from the hippocampi in sham (a, f, k), and rats subjected to 5 days of reperfusion after 15 min of ischemia with saline control (d, I, n), administration of the Rac1 missense ODNs (b, g, l) and Rac1 AS-ODNs (c, h, m) or Rac1 inhibitor NSC23766 (e, j, o) before ischemia. (B) The number of survival CA1 neurons per 1mm length of the medial CA1 pyramidal cell layer was counted as indicated. Note that treatment with Rac1 AS-ODNs or NSC23766 significantly increased the number of the survival neurons compared with control at 5 days of reperfusion in the CA1 region. Data were obtained from five independent animals and a typical experiment is presented. Boxed areas in left column are shown at higher magnification in right column.*p < 0.05 versus sham control. # p < 0.05 versus MS or saline group. (a–e) magnification is 5° and the scale bar = 200 μm; (f–o) magnification is 40° and the scale bar = 50 μm.