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Splenectomy strategy. Splenectomy or sham surgery was performed on op/op and control mice. Seven days later, mice were injected with 250 μg/kg per day granulocyte colony-stimulating factor daily for 5 d and hematopoietic stem cell mobilization to peripheral blood was analyzed using flow cytometry and colony-forming assays. G-CSF: Granulocyte colony-stimulating factor.
