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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Immunol. 2013 Aug 30;191(7):3838–3846. doi: 10.4049/jimmunol.1300524

Figure 4.

Figure 4

Activation of mTOR/p70 S6 kinase involved in the H. pylori mediated downregulation of B7-H2 expression. N87 cells were incubated with H. pylori 51B WT and cagA strain. Cell were lysed after 5 min, 15 min, 30 min, 45 min and 60 min. Cell lysates from GEC exposed to H. pylori 51B WT and cagA strain was analyzed for (A, C) phospho and (B, D) total p70 S6 kinase using Luminex bead array respectively. (E) GECs were treated with rapamycin (100 ng/ml) an inhibitor of mTOR/p70 S6 kinase pathway, (F) or with wortmannin (100 nM), AG-490 (100 ng/mL) and CAY10512 (10 μM) inhibitor for PI3K, STAT3 and NFκB pathway respectively, for 1 hour and then were infected with H. pylori. B7-H2 expression was analyzed using flow cytometry 24 hour later. Data expressed as a percentage of positive cells. Results represents as the mean ± SD (n=8); P=N.S. (Not significant); *P < .05.