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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Immunol. 2013 Aug 30;191(7):3838–3846. doi: 10.4049/jimmunol.1300524

Figure 6.

Figure 6

H. pylori mediated downregulation of B7-H2 on GEC in vivo involves CagA function and inversely correlate with the bacterial clearance. (A) C57BL/6 mice were challenged with H. pylori strain PMSS1, which express functional T4SS and can deliver CagA or with H. pylori SS1, which does not. Gastric mononuclear cells were isolated four weeks after H. pylori challenge using enzymatic digestion and expression of B7-H2 and epithelial cell marker EpCAM was analyzed by flow cytometry. (B) Level of the B7-H2 expressing epithelial cells (EpCam+) in the gastric mucosa from the cells was measured by flow cytometry (C) Infection rate was determined by quantification of H. pylori genome copy per half of stomach based on the analysis of H. pylori 16S gene amplification by real time PCR. Each data point represents a single mouse tested in quadruplicate. Average bar of infection rate were calculated from five mice per group and demonstrated as a mean ± SD.