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Regulation of MMP9 activation in retinal endothelial cells does not protect H-Ras activation. Cells transfected with MMP9 siRNA (MMP-si) or scramble RNA (SC) were incubated in 5 mM or 20 mM glucose for 4 days. The effect of MMP9 regulation on H-Ras activation was determined by (a) pull-down assay, and (b) by quantifying its protein expression. *P < 0.05 compared with values obtained from cells incubated in 5 mM glucose.
