Skip to main content
. Author manuscript; available in PMC: 2014 Oct 3.
Published in final edited form as: Neuron. 2013 Oct 2;80(1):72–79. doi: 10.1016/j.neuron.2013.07.020

Figure 3. Group 1 mGluR triggered LTD in ArcGFP+ neurons requires de novo protein synthesis and Arc and occurs in vivo with repeated novelty experience.

Figure 3

A) Anisomycin (25 µM) blocks DHPG-induced decreases in mEPSC frequency (Avg±SEM) in ArcGFP+ neurons (n=13) from novelty exposed ArcGFP-BAC mice. B) Novelty-exposed heterozygous ArcGFP-KI+/− mice exhibit DHPG-induced LTD of mEPSC frequency in ArcGFP+, but not ArcGFP– neurons (n=9,12). C) DHPG-induced LTD of mEPSCs is absent in ArcGFP+ neurons of homozygous ArcGFP-KI −/− (ArcKO) mice (n=11). D)ArcGFP+ neurons from ArcGFP-BAC mice exposed to repeated experience of the same environment display reduced mEPSC frequency in comparison to neighboring ArcGFP– neurons (n=10 cell-pairs) E) DHPG has no effect on mEPSCs in ArcGFP+ neurons from ArcGFP-BAC mice with repeated experience (n=9). For Figures 3A–3E: left panels depict group averages (±SEM) of mEPSC frequency and amplitude before and 30 min after DHPG and right panels depict representative raw and average mEPSCs at baseline and 30 min after DHPG for ArcGFP+ (black) and ArcGFP– (grey) neurons. Scale-bars: 1s/10 pA and 10 ms/2 pA. *p< 0.05, **p< 0.01. F) Proposed model of in vivo Arc induction by novelty experience and LTD priming. See text and Figure S3.