Figure 7.

In vitro expanded organoids give rise to Glucagon (GCG) and Somatostatin (SST) mono-hormonal cells in vivo. Pancreas organoid cultures derived from sorted eGFP⁺ cells and expanded for at least 2 months in culture were grafted as described in Figure 6. Two weeks after transplantation, the kidney grafts were harvested and the grafted eGFP⁺ cells were evaluated for the expression of glucagon (GCG) and Somatostatin (SST). (A, B) Representative images illustrating that eGFP⁺ cells (green) differentiate towards GCG⁺ (A) or SST⁺ (B) cells in vivo. Note that both Gcg⁺ and SST⁺ cells do not express INS (insulin, blue), indicating that the organoid-derived eGFP⁺ cells have fully differentiated into mono-hormonal cells in vivo. Scale bars=20 μm.