Figure 8.

Effect of absence of CD28 on the development of OVA-induced eosinophilic airway inflammation. On day 0, CD28^+/+ and CD28^–/– mice received 5 × 10⁵ OVA-DC from wild-type mice. Mice were subsequently exposed to OVA aerosol from day 14 to day 20 and BALF was recovered 24 hours after the last aerosol challenge. (a) Differential cell counting on BALF. Results represent means ± SEM from six mice per group. (b) Intracellular staining for cytokines on BALF T cells. Cells were restimulated with PMA/ionomycin in the presence of monensin and stained for IFN-γ and IL-4. Percentage expression of each cytokine on CD3⁺CD4⁺ cells is indicated.