Figure 5.

Effects of glucose and insulin on cultured angioblasts. The number of spindle-shaped cells were counted in cultures of freshly isolated CD34⁺ cells plated on fibronectin at various times after plating. Results from four to six experiments at each time point, performed at least in duplicate, are pooled. (a) Cells cultured in medium or medium with 30 mM glucose. Data are presented as percentage of spindle-shaped cells in glucose-treated dishes relative to untreated controls. (b) Cells cultured in medium or medium with 6–12 μg/mL supplemental insulin. Data are presented as percentage of spindle-shaped cells in insulin-treated dishes relative to untreated controls. Error bars indicate standard errors for both a and b.