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. Author manuscript; available in PMC: 2014 Sep 11.
Published in final edited form as: Cell Host Microbe. 2013 Sep 11;14(3):281–293. doi: 10.1016/j.chom.2013.08.002

Figure 4. High free cholesterol pools in Niemann-Pick Type C disease fibroblasts stimulate replication.

Figure 4

(A) Free cholesterol distribution in wild type and NPC2 (−/−) fibroblasts.

(B) PV replicon replication in wild type and NPC2 (−/−) fibroblasts. Mean data +/−SEM with 6 replicates each is plotted.

(C) Peak PV replication levels for wild type, NPC1 (−/−) and NPC2 (−/−) fibroblasts. Mean data +/− SEM from 5 independent experiments, normalized by the expression of reporter luciferase RNA, is plotted. ***: p<0.001

(D) Cholesterol and viral dsRNA distribution in wild type and NPC2 (−/−) fibroblasts.

(E) Free cholesterol levels within wt and NPC2 (−/−) fibroblast replication organelles. Mean data +/−SEM, from wild type (n=30) and NPC2 (−/−) (n=30) fibroblasts plotted.

(F) Acute cholesterol extraction inhibits PV replicon replication in NPC2−/− fibroblasts. Mean data +/− SEM from 2 independent experiments is plotted.

(G) 3CDpro / 3Dpol ratio in wild type and NPC2 (−/−) fibroblasts. Mean data +/−SEM from 3 independent experiments are plotted.

Scale bars 10µm.

Figure 4 related to Figure S4.