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. Author manuscript; available in PMC: 2013 Oct 21.
Published in final edited form as: Biomed Chromatogr. 2010 Jan;24(1):29–38. doi: 10.1002/bmc.1374

Figure 10.

Figure 10

Evaluation of ion suppression on response of LTE4 and LTE4-d3 in urine and water. Injection experiments: extracted ion chromatogram (EIC) of MRM transitions for LTE4-d3 (m/z 443.2 → 304.3) spiked at 200 pg/mL in a human urine sample (A) and water (B), and MRM transition of endogenous LTE4 (m/z 440.2 → 301.3) in a human urine sample (C, hatched lines). Data demonstrate that the response of internal standard is not affected by ion suppression. Data was collected in positive electrospray ionization mode. The peak area for urine and water spiked with LTE4-d3 was 115754 and 107672, respectively. Post-column infusion experiments: LTE4 was infused and the 440.2 → 301.2 transition was monitored during injection of urine (D) and water (E) samples. Data demonstrate the absence of ion suppression in a urine matrix. Reprinted with permission from Armstrong et al. (2009).