Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Schematic of selected reporter plasmids and controls used (see Table S1, Supporting Information, and text). Regions cloned upstream of the promoterless luxCDABE cassette are indicated by a thick line. pTIM5319 carries a truncated lasR in which the AHL binding pocket (S13-S172, indicated by a thin dashed line) was removed using primers GCGTGGCGATGGGCCGACAGTG and GCGTTCCAGCTCAAGAAAACCGTC; AAACCGGTGGTTCTGACCAGCCGG and CACTAACGTCCCAGCCTTTGCGCTC, as described in Rajamani et al., 2008.34
