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. Author manuscript; available in PMC: 2013 Oct 21.
Published in final edited form as: Mol Biosyst. 2011 Jan 24;7(4):1205–1216. doi: 10.1039/c0mb00180e

Figure 5.

Figure 5

Probing of the mechanism of inhibition by 4 by use of luxCDABE reporter constructs, and comparison of 4 with compounds of related structure. (*) Indicates downregulation with statistical significance of P < 0.05 (t-test), compared with untreated controls, and error bars depict standard deviation. a) Effect of lyngbyoic acid (4) and dodecanoic acid (9) on a reporter that lacks a functional AHL-binding domain (pTIM5319).34 Cultures of the reporter were grown in 96-well plates in the presence of varying concentrations of 4 or 9. Results are expressed as % activation compared to untreated control (set to 100%). b) 3-oxo-C12-HSL is able to compete with 4 (1 mM) in a reporter strain lacking rsaL (pTIM505 5211), but not in pSB1075. c) Effect of lyngbyoic acid (4) and other compounds on luminescence in pSB1075.