Skip to main content
. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Neurotoxicology. 2013 Jul 8;38:91–100. doi: 10.1016/j.neuro.2013.06.008

Fig. 2.

Fig. 2

Low dose MeHg enhances CNTF-evoked STAT3-phosphorylation. SH-SY5Y cells (A) and NPCs (B) were stimulated with 20 ng/mL CNTF for 30 min following 5 h toxicant exposure. P-Y705 STAT3, Total STAT3, and β actin levels in whole cell lysates were determined using western blots (upper three rows in each panel). The extent of STAT3 phosphorylation was quantified by densitometry, normalized to total STAT3 level and standardized to the value observed in cells treated with CNTF alone (bar graphs). HgCl2 inhibited STAT3 phosphorylation by CNTF whereas STAT3 phosphorylation was significantly enhanced 155-170% by ⎕ ⎕ ⎕ ⎕ ⎕̣ ⎕ ⎕ ⎕ ⎕ ⎕̣ ⎕ ⎕ MeHg and inhibited at higher doses. Data are mean +/- SEM from at least 3 independent replicates per condition. * = p < 0.05, ** = p < 0.01 significantly different from positive control by ANOVA and post hoc Bonferroni's test.