FoxM1 expression and its effects on cell growth after DIM and Taxotere treatment. For single-agent treatment, breast cancer cells were treated with DIM (5, 10, 25, or 40 μM), Taxotere (0.5, 1.0, or 1.5 nM) alone for 72 hr (a and b). For combination, breast cancer cells were treated with 25 or 40 (MDA-MB-231 only) μM of DIM and then exposed to 1.0 nM of Taxotere for 72 hr (c). DIM in combination with Taxotere significantly inhibited cell proliferation in all breast cancer cells (c) as measured by MTT assay. FoxM1 expression was evaluated by real-time RT-PCR and Western blotting (d). FoxM1 mRNA levels were downregulated by DIM, followed by Taxotere treatment as measured by real-time RT-PCR (e–h). Tax, Taxotere. β-actin protein was used as protein loading control for Western blot, whereas GAPDH was used as internal control for the real-time RT-PCR analysis. The data was obtained from three individual experiments. *, p < 0.05; **, p < 0.01 relatively control.