Figure 4.

TNF-α–induced upregulation of NF-κB in ONH astrocytes. ONH astrocytes were starved overnight in serum-free medium. Cells were then pretreated with SNC-121 (1 μmol/L) for 15 minutes followed by TNF-α (25 ng/mL) treatment for 6 (A) or 24 (B) hours. Cell lysate (15 μg protein) was analyzed by Western blotting using selective anti–NF-κB antibodies followed by incubation with appropriate secondary antibodies (HRP-conjugated; dilution 1:3000). The signal was captured using enhanced chemiluminescent reagent and a commercial imaging system (Bio-Rad). Data are expressed as mean ± SE. *P < 0.05. n = 4.