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. 2013 Oct 9;54(10):6612–6619. doi: 10.1167/iovs.13-12861

Figure 3.

Figure 3

Quantitative reverse transcriptase PCR analysis of both HCKs and HCFs for (A) Col I, (B) Col V, and (C) Col III expression. Four conditions were tested: No VitC, VitC, T3, and VitC+T3. HCKs showed significant upregulation of Col I and V (∼4–6-fold, P < 0.01) when T3 was present independent of VitC stimulation [A] and [B], respectively). On a smaller scale HCFs also showed upregulation of both Col I and V (∼2-fold, P < 0.05). HCKs expressed Col I and V at higher levels compared with HCFs under all conditions. Col III (C), on the other hand, was significantly upregulated in HCFs under all conditions when compared with HCKs (∼6–53-fold, P < 0.01).