Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Feb 7;22(10):2083–2096. doi: 10.1093/hmg/ddt058

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 9. — AF-6 is decreased in PD patients and aggregates into Lewy bodies. (A) Homogenates prepared from different rat brain regions were probed with an anti-AF-6 antibody (upper panel). Loading control was monitored with an anti-actin antibody (lower panel). (B) Homogenates prepared from control and PD substantia nigra tissues were probed with an anti-AF-6 antibody (upper panel). Loading control was monitored with an anti-actin antibody (lower panel). (C) The graph represents the average of AF-6 levels in the substantia nigra shown in (B). Levels of proteins were determined by ImageMaster analysis and AF-6 were normalized relative to actin. Error bars represent standard error of four different patients. **Significantly different from control at P < 0.01. (D) Homogenates prepared from control and PD caudate and putamen tissues were probed with an anti-AF-6 antibody (upper panel). Loading control was monitored with an anti-actin antibody (lower panel). (E) The Graph represents the average of AF-6 levels in the caudate and putamen shown in (D). Levels of proteins were determined by ImageMaster analysis and AF-6 were normalized relative to actin. Error bars represent standard error of four different patients. **Significantly different from control at P < 0.01. (F) Immunohistochemistry analysis of midbrain sections incubated with a purified anti-AF-6 antibody. Lewy bodies containing AF-6 are shown in sections from two distinct PD cases (left panels). Right panels show the presence of AF-6 in Lewy neurites. Scale bar is 20 μm.