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Determination of the accuracy of the PheT analysis. A pooled smokers’ urine sample containing 2199 fmol/mL PheT was spiked with 6 increasing amounts of PheT and the analysis was carried out as described in Materials and Methods. Each point is the mean of 4 determinations. The added and determined amounts correlated, R2 = 1.00, and the accuracy was 95%. The y-intercept agrees with the amount of PheT in the pooled smokers’ urine sample.
