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. Author manuscript; available in PMC: 2013 Oct 21.
Published in final edited form as: Cell Rep. 2013 Aug 22;4(4):724–737. doi: 10.1016/j.celrep.2013.07.028

Figure 4. Lentiviral over-expression of CREB induces the expression of plasticity-related molecules and restores calcium influx in Ppara-null hippocampal neurons.

Figure 4

(A) E18 Ppara-null hippocampal neurons were transduced with control (CLV) and CREB (*CREB) lentivirus at a MOI of 10. After 48 h, the expression of CREB protein was monitored in untransduced, CLV, and CREB-transduced neurons by Western blot. (B) Densitometric analyses of CREB expression. Results are mean ± SD of three independent results. ap< 0.005 vs. CLV. (C) Immunoblot analyses of NR2A and GluR1 in untransduced, CLV, and CREB lentiviral-transduced Ppara-null hippocampal neurons. (D) Densitometric analysis of NR2A and GluR1. Results are mean ± SD of three different experiments. ap< 0.005 and bp<0.01 vs. NR2A and GluR1, respectively in CLV-transduced neurons. Calcium influx in WT, Ppar-null, CLV, and CREB- transduced Ppara-null neurons was assayed in the presence of (E) NMDA and (F) AMPA. Results are mean ±SD of three independent experiments.