FIGURE 5.

Psychosine-induced “globoid-like cell” formation in vitro requires MMP-3. Primary mixed glial cultures were prepared from MMP-3 KO and WT littermates and then treated with psychosine (10 μM) for 7 days. ICC was performed using Iba-1 antibody (green) and DAPI (blue) and the number of multinucleated microglia were quantified. The numbers of Iba-1+ cells did not differ between untreated weight of KO cultures. The prevalence of globoid-like cells observed in weight cultures treated with psychosine was elevated sixfold over vehicle treatment (A–D) but were absent from the MMP-3 KO cultures (D). Data represent n = 8/treatment; **P < 0.01, t-test versus WT. Scale bar = 20 μm. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]