Fig. 2.

Generation of a Gal3/1ctR chimera. The Gal3/1ctR chimera, for which “1ctR” refers to the carboxy tail of GalR1, was generated by in-frame fusion of wild-type GalR3 (residues 1–299) with the carboxy tail of GalR1 (residues 299–350) as described in Materials and Methods. Removal of the arginine-rich putative endoplasmic reticulum (ER)-retention motifs (highlighted in green) found in the carboxy tail of wild-type GalR3 was proposed to enable plasma membrane expression of the receptor. The highly conserved characteristic NPxxY motif found in the seventh transmembrane domain of most GPCRs is highlighted in red.