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. Author manuscript; available in PMC: 2013 Oct 21.
Published in final edited form as: Nat Neurosci. 2012 Nov 11;15(12):1655–1666. doi: 10.1038/nn.3259

Figure 1.

Figure 1

CaMKII activity is required for both Bic-induced removal and TTX-dependent accumulation of GKAP and PSD-95 at synapses.

(a,b) Changes in the GKAP and PSD-95 clusters 24 h after treatment with either (a) TTX (2 μM) alone, TTX + KN-93 (10 μM), or TTX + KN-92 (10 μM), or (b) Bic (40 μM) alone, Bic + KN-93 (10 μM), or Bic + KN-92 (10 μM). (c) Effect of KN-93 or KN-92 alone on GKAP and PSD-95 clusters.

(d,e) Quantification of GKAP and PSD-95 cluster density (d) and intensities (e) 24 h after the indicated treatments. Each bar represents mean ± s.e.m. n ≥ 20 neurons per condition.***P < 0.001 compared to Control. (f) Effect of AP5 (100 μM) and Nimo (5 μM) on Bic- or TTX-induced changes of GKAP and PSD-95 clusters at synapses. (g) Quantitative analysis shows effect of the Ca2+ channel blockers on the density of GKAP and PSD-95 clusters. Each bar represents mean ± s.e.m. n ≥ 20 neurons per condition.

*** P < 0.001. Scale bars, 5 μm.