Skip to main content
. Author manuscript; available in PMC: 2013 Oct 21.
Published in final edited form as: Nat Neurosci. 2012 Nov 11;15(12):1655–1666. doi: 10.1038/nn.3259

Figure 7.

Figure 7

GKAP (ΔR1) mutant blocks activity-dependent remodeling of PSD proteins. Hippocampal neurons (14 DIV) were transfected with HA-tagged WT or ΔR1 GKAP. After 1 d post-transfection, neurons were treated with either Bic (40 μM) or TTX (2 μM), and incubated further 24 h (for PSD-95 and Shank) or 48 h (for GluRs) before fixation and immuno-staining for the indicated proteins. (a) Effect of GKAP (WT) or GKAP (ΔR1) overexpression on the activity-dependent changes in endogenous PSD-95. (b) Quantification of activity-dependent changes of transfected WT or ΔR1 mutant GKAP, measured by HA cluster intensity (normalized to WT control level). n > 20 per condition. (c,d) Quantification of activity-dependent changes of endogenous PSD-95 (c) and Shank (d) in Non-txf, WT GKAP-, or ΔR1-transfected neurons. n > 20 per condition. (e) Effect of GKAP (WT) or GKAP (ΔR1) overexpression on the activity-dependent changes in the phosphorylation of Ser-295 (pS-295) of PSD-95. (f) Quantification of activity-dependent changes in the amount of pS-295 of PSD-95. n ≥ 20 per condition. *** P > 0.001, n.s., not significant. (g) Effect of GKAP (WT) or GKAP (ΔR1) overexpression on the activity-dependent changes in surface expression of GluR1 (sGluR1). (h) Quantification of activity-dependent changes in surface expression of AMPA receptors. n ≥ 20 per condition.*** P > 0.001, n.s., not significant. Scale bars, 5 μm. Error bars represent s.e.m.