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. Author manuscript; available in PMC: 2014 Jan 24.
Published in final edited form as: Neuron. 2013 Jul 24;79(2):241–253. doi: 10.1016/j.neuron.2013.05.022

Figure 5. The Extent of Singlet Oxygen-Mediated Photo-oxidation as Measured with IFP Photobleaching.

Figure 5

(A) Schematic drawing showing the three conditions tested in the IFP bleaching experiments. IFP was expressed in neurons either fused to the C terminus of the SYP1 or SYP1-miniSOG (left panel). In the other conditions, IFP was coexpressed as synaptotagmin-1 (SYT1) fusion (middle panel) or tethered to the plasma membrane (pm-IFP) in neurons expressing SYP1-miniSOG-Citrine or SYP1-Citrine.

(B) Summary graph showing the IFP bleaching in the six conditions tested. Significant greater IFP bleaching were observed in all three conditions. A smaller difference was observed when IFP was expressed on the plasma membrane and SYP1-miniSOG or SYP1 were expressed on the vesicles. MiniSOG was excited by 93 s of 495 nm light at 20 mW/mm2 and IFP was imaged with 665 nm excitation light.

*** indicates difference at p < 0.001. The error bars indicate SEM.