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. 2013 Oct 17;8(10):e77677. doi: 10.1371/journal.pone.0077677

Figure 2. Reduced mast cell numbers in vitro but normal mast cell homeostasis in vivo.

Figure 2

(A) Diagram showing the cumulative numbers of c-kit+FcεRI+ Mazr F/F and Mazr F/F Vav-iCre BMMC over the course of 5 weeks of culture. Cells were counted by CASY counter, then percentages of c-kit+FcεRI+ BMMC among PI-negative cells (= alive) was determined by flow cytometry. The summary of three independent experiments with a total of 6 independent cell batches is shown. Mean ± SEM is shown.

(B) Number of PI-negative (= alive) Mazr F/F and Mazr F/F Vav-iCre BMMC during 5 days of IL-3 starvation. The summary of 3 experiments is shown. Mean ± SEM is shown.

(C) Toluidine blue staining of paraffin-embedded 5 µm ear sections of Mazr F/F and Mazr F/F Vav-iCre mice showing mast cells in pink/purple color (examples indicated by arrowheads). Diagram at the right indicates mean ± SEM of mast cell number per field of view (fov) calculated over 10 individual sections per ear (n=4). Magnification 20x.

(D) Percentage of EYFP+c-kit+FcεRI+ mast cells from peritoneal lavage of wild-type (MazrF/+Rosa26+/EYFPMcpt5Cre) and mast cell-specific MAZR-null (MazrF/FRosa26+/EYFPMcpt5Cre) mice (n=8 and 9, respectively).