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. 2013 Sep 23;191(9):4769–4777. doi: 10.4049/jimmunol.1301653

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Copyright © 2013 by The American Association of Immunologists, Inc.

This article is distributed under The American Association of Immunologists, Inc., Reuse Terms and Conditions for Author Choice articles.

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FIGURE 1. — Expression of recombinant IgG2/4 hybrid Abs. (A–C) The Ab concentration in cell culture supernatant (μg/ml; ○) and the Ab production rate (pg/cell/day; ●) during a fed-batch expression period of 12 d were determined with ELISA. Data are given as mean and SEM for rMil2 (A; n = 12), r18D11 (B; n = 5) and raNIP (C; n = 2). (D) Five hundred nanograms of each purified Ab was subjected to either reducing or nonreducing SDS-PAGE and stained with Coomassie Blue. IgG2/4 isotype controls raNIP and anti-human C5 Ab eculizumab (ctrl IgG2/4) were included. (E) The same samples (10 ng) were subjected to nonreducing SDS-PAGE and immunoblotting using an Ab specific for the human IgG2 hinge region.