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. 2013 Sep 23;191(9):4769–4777. doi: 10.4049/jimmunol.1301653

FIGURE 3.

FIGURE 3.

Effect of rMil2 in combination with C5-inhibitor OmCI on the inflammatory response in porcine blood in vitro. Porcine whole blood was incubated with E. coli in the presence of PBS (E. coli), the complement C5 inhibitor OmCI (0.32 μM), original anti-CD14 Ab Mil2 (25 μg/ml), recombinant anti-CD14 Ab rMil2 (25 μg/ml), the combination of OmCI and the two Mil2 Abs, or an isotype control Ab. Plasma was analyzed for cytokines. (A) TNF. (B) IL-1β. (C) IL-8. (D) TF expression on granulocytes was measured by flow cytometry and expressed as median fluorescence intensity (MFI). Results are shown as mean ± SEM of four to eight experiments. #Statistical significance between combined inhibition versus the two single inhibitions (for detailed p values, see Results). *Statistical significance as compared with E. coli alone (left column). *p < 0.05, **p < 0.01, ***p < 0.001.