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. 2013 Jun 14;22(21):2815–2824. doi: 10.1089/scd.2012.0596

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Copyright 2013, Mary Ann Liebert, Inc.

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FIG. 2. — Hypoxia inhibits TS cell differentiation. WT and PPARγ-null TS cells were differentiated under normoxia or hypoxia (2% oxygen); qPCR was done for both giant cell markers (A) or labyrinthine/STB markers (B). Data are expressed as fold change over WT-Stem. * Indicates statistically significant difference between levels in normoxia and hypoxia for the indicated cell line. (C) Morphology of WT and PPARγ-null TS cells differentiated in either normoxia or hypoxia. Cells were stained with phalloidin and dapi. Arrowheads point to giant cells and * indicates multinucleated STB. Bar=100 μm. STB, syncytiotrophoblast.

FIG. 2. — Hypoxia inhibits TS cell differentiation. WT and PPARγ-null TS cells were differentiated under normoxia or hypoxia (2% oxygen); qPCR was done for both giant cell markers (A) or labyrinthine/STB markers (B). Data are expressed as fold change over WT-Stem. * Indicates statistically significant difference between levels in normoxia and hypoxia for the indicated cell line. (C) Morphology of WT and PPARγ-null TS cells differentiated in either normoxia or hypoxia. Cells were stained with phalloidin and dapi. Arrowheads point to giant cells and * indicates multinucleated STB. Bar=100 μm. STB, syncytiotrophoblast.