Expression of Cx43 and Cx40. RT-PCR analysis of Cx mRNA expression (A) in A7r5 and pSMCs cells demonstrates a higher expression of Cx43 in pSMCs than in A7r5 cell, and the absence of Cx40 in pSMCs. The mean value of A7r5 cells, control, is presented as 1. Western blot analysis in (B) and densitometric analysis of Cx protein levels in (C) also showed the higher expression of Cx43 and the absence of Cx40 in pSMCs. 6B5N cells express the same amount of Cx43, but lower Cx40 compared to A7r5 cells. Densitometric data were normalized to A7r5 cells, separately for each connexin type.